We hypothesized that species that overlap in calling sites partition the acoustic space, whereas those that segregate calling sites overlap in the acoustic space. Most of the 12 analyzed species (75%; n = 9 species) were generalists with respect to at least one of the calling site variables, a characteristic of species living in unpredictable or human-altered habitats. In most water bodies, segregation in calling sites occurred as a result of low species richness in the communities. Higher overlaps in calling sites were observed among species that called from the ground or while floating in water than among those that called perched on vegetation, probably due to differences in the use of two- versus three-dimensional spaces. As a result of the analysis of similarity among advertisement call features, we observed higher segregation among species that called on the ground or floating on water than perched on vegetation. For those species, differences in advertisement calls seem to explain their coexistence.